Bone and Cartilage Cellular and Molecular Biology Facility

Bone and Cartilage Cellular and Molecular Biology Facility

The cell biology laboratory is fully equipped for cell culture and routine biochemical and molecular biology procedures.

Lab Technician prepares cell cultures in biological safety cabinet
Lab Technician prepares cell cultures in biological safety cabinet

Cell culture facilities are comprised of six O2 and CO2 controlled incubators, two biological safety hoods, a Ruskinn INVIVO 300 workstation, two tissue culture microscopes, freezers (-80C, -20C and 4C) as well as liquid nitrogen tanks, and bench top and refrigerated centrifuges.

 


 

Dr. Yellowley taking immunofluorescent images
Dr. Clare Yellowley takes direct immunofluorescence images

The microscopy area houses a state of the art microspectrofluorometry system (inverted NIKON Eclipse TE 2000-U microscope with epifluorescence and a Sutter filter switcher), equipped for quantifying cytosolic ion concentration, fluorescent dye transfer and image analysis using Metamorph. In addition an upright NIKON Eclipse E600 microscope is also equipped with epifluorescence and imaging software for immunocytochemical and immunohistochemical assays. For experiments requiring cells grown in dishes, a LEICA AF6500 inverted fluorescent microscope (with Image-Pro Plus software) is also available.

 

student running PCR
PhD student runs PCR

Also located within the laboratory are the following: pH meter, thermal cycler for PCR, luminometer, power supplies and equipment for electrophoresis, Bio-Tek Synergy HT plate reader (with visible, fluorescent and luminescence capabilities), walk in cooler, dissection/necropsy room, mechanical testing machine (Enduratec Test bench), ultrapure water system, two fume hoods, and water baths. The lab has recently been outfitted with an Eppendorf Mastercycler EP Realplex real-time PCR system and Biophotometer for analysis of DNA, RNA, and protein, as well as a dedicated bioimaging system for quantitation of Western chemiluminescence, fluorescence and ethidium bromide.